Role of the TM
The trabecular meshwork (TM) is the primary outflow pathway, responsible for up to 80% of aqueous outflow humor, followed by the uveoscleral outflow pathway.1 Nitric oxide deficiency is believed to play a role in chronic TM contraction.2
Glaucomatous eyes have shown a ~40% reduction in nitric oxide markers compared with normal eyes.3
VYZULTA is the only PGA that delivers nitric oxide as part of a dual MOA with latanoprost acid to increase outflow and reduce IOP.4,5
Nitric oxide targets the primary outflow pathway to improve TM permeability.5
Latanoprost acid opens the interstitial spaces of the uveoscleral pathway.5
Go deeper into the dual MOA of VYZULTA and how nitric oxide inhibits both rho kinase and calcium signaling to relax the TM.6,7
Facilitating outflow through cellular relaxation
Human TM cells were stained for F-actin (green) to assess for stress fiber formation and cellular contraction.5
Normal TM cells
Contracted TM cells
Contracted TM cells + latanoprost
Contracted TM cells + latanoprostene bunod
Notable reduction of F-actin in human TM cells with latanoprostene bunod vs latanoprost.*
*The clinical significance of in vitro data is unknown.
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VYZULTA® (latanoprostene bunod ophthalmic solution), 0.024% is indicated for the reduction of intraocular pressure (IOP) in patients with open-angle glaucoma or ocular hypertension.
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References: 1. Winkler NS, Fautsch MP. Effects of prostaglandin analogues on aqueous humor outflow pathways. J Ocul Pharmacol Ther. 2014;30(2-3):102-109. 2. Aliancy J, Stamer WD, Wirostko B. A review of nitric oxide for the treatment of glaucomatous disease. Ophthalmol Ther. 2017;6(2):221-232. 3. Galassi F, Renieri G, Sodi A, Ucci F, Vannozzi L, Masini E. Nitric oxide proxies and ocular perfusion pressure in primary open angle glaucoma. Br J Ophthalmol. 2004;88(6):757-760. 4. VYZULTA. Prescribing Information. Bausch & Lomb Inc. 5. Cavet ME, Vollmer TR, Harrington KL, VanDerMeid K, Richardson ME. Regulation of endothelin-1-induced trabecular meshwork cell contractility by latanoprostene bunod. Invest Ophthalmol Vis Sci. 2015;56(6):4108-4116. 6. Suzuki H, Kimura K, Shirai H, et al. Endothelial nitric oxide synthase inhibits G12/13 and rho-kinase activated by the angiotensin II type-1 receptor: implication in vascular migration. Arterioscler Thromb Vasc Biol. 2009;29(2):217-224. 7. Dismuke WM, Mbadugha CC, Ellis DZ. NO-induced regulation of human trabecular meshwork cell volume and aqueous humor outflow facility involve the BKCa ion channel. Am J Physiol Cell Physiol. 2008;294(6):C1378-C1386.
VYZULTA® (latanoprostene bunod ophthalmic solution), 0.024% is indicated for the reduction of intraocular pressure (IOP) in patients with open-angle glaucoma or ocular hypertension.